枸杞多糖对己烯雌酚染毒仓鼠睾丸组织Bax及Bcl-2凋亡蛋白的影响

doi:10.11843/j.issn.0366-6964.2014.08.023

Abstract

Abstract:

The current study was undertaken to study the protective effects of lycium barbarum polysaccharides (LBP) on the expression of Bax and Bcl-2 in hamster testicular tissue treated by diethylstilbestrol.Sixty-six adult male hamsters were randomly divided into the control group (A)，DES group (B)，1，10，50 mg•kg^－1 LBP group (C，D，E) and V_C+V_E group (F).Except the control group，the other 5 groups were subcutaneous injected with DES for 7 consecutive days，while the control group was injected equivalent amount of olive.Meanwhile hamsters in C，D，and E groups were gavaged 1，10，50 mg•kg^－1 LBP respectively，and the group of F were gavaged 100 mg•kg^－1 V_C+200 IU•kg^－1 V_E，while the groups of A and B were given equivalent amount of normal saline.After 24 hours of the last treatment，the expressions of Bcl-2 and Bax in testicular tissue were tested through RT-PCR and Western blot.Results showed that DES dramatically decreased the expression of Bcl-2 (P<0.01)，but the expression of Bax significantly increased.After treating with different dosage of LBP，the expression of Bcl-2 increased in a dose dependent manner，while the expression of Bax decreased，with 50 mg•kg^－1 LBP being the best (P<0.01).There was no significant difference between vitamin group and 50 mg•kg^－1 LBP group.These results indicated that regulating the expression of Bax and Bcl-2 may be one of the pathways that LBP alleviating the spermatogenic damage induced by DES on adult male hamster.

CLC Number:

S853.74

ZHANG Cai-li，LIU Yu-jiao，ZHAO Xing-hua，MA Ai-tuan. Effects of Lycium Barbarum Polysaccharides on the Expression of Bax and Bcl-2 in Hamster Testicular Tissue Treated by Diethylstilbestrol[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(8): 1363-1368.

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Effects of Lycium Barbarum Polysaccharides on the Expression of Bax and Bcl-2 in Hamster Testicular Tissue Treated by Diethylstilbestrol

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